Drako Bar was closed for the second time since two people were killed in the venue last year. The bar had illegally reopened after its first closure.
Other nightclubs and bars were closed for failing to meet the necessary security standards, or for operating at unlicensed hours.
Guadalajara Mayor Enrique Alfaro has quickly established a reputation as a regulator, and the sanctioning of nightclubs is the latest in a long series of crackdowns.
With an eye of the governor’s job for 2018, Alfaro has been enthusiastically enforcing legislation affecting street vendors, valet parking attendants and drivers with tinted windows.